The opposite of dest in lower.keys() is dest not in lower.keys(). No need to compare to False or True.
in operator: The ‘in’ operator is used to check if a value exists in a sequence or not. Evaluates to true if it finds a variable in the specified sequence and false otherwise.
‘not in’ operator: Evaluates to true if it does not find a variable in the specified sequence and false otherwise.
In your case, this will work well -
if (dest not in lower.keys()):