While it is possible to use React Testing Library without Jest, it is highly recommended to use them together. React Testing Library is designed to work seamlessly with Jest because Jest provides built-in functionality such as a test runner, mocking, and assertions.
If you choose not to use Jest, you'll need to configure another testing framework like Mocha or Ava alongside a suitable assertion library. However, this requires more setup and might not be as streamlined as using Jest with React Testing Library.