If you can comment out code and your program still works, then yes, that code was optional.
.strip() with no arguments (or None as the first argument) removes all whitespace at the start and end, including spaces, tabs, newlines and carriage returns. Leaving it in doesn't do any harm, and allows your program to deal with unexpected extra whitespace inserted into the file.
For example, by using .strip(), the following two lines in a file would lead to the same end result:
foo\tbar \n
foo\tbar\n